Expression of Cold-Inducible Proteins in Rat Spinal Cord Subjected to Systemic Hypothermia

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Aníbal José Sarotto
Manuel Rey-Funes
Verónica Berta Dorfman
Daniela Contartese
Ignacio M. Larráyoz
Alfredo Martínez
María Agustina Toscanini
César Fabián Loidl

Abstract

Introduction: Traumatic spinal cord injury is the main cause of motor disability in developed and underdeveloped countries, being a priority interest to the WHO. The effect of hypothermia on the expression of CIRBP (cold-activated protein) in the anterior grey column of 60-day-old male albino Sprague-Dawley rats was studied at the structural and biochemical levels and proposed as a possible therapeutic approach.
Materials and Methods: 24 rats were randomly divided into two groups; normothermia (n = 6), at 24º C, and hypothermia, (n = 18) at 8º C for 180 minutes and euthanized at 12, 24, and 48 h post-treatment. Western blot and immunohistochemistry for CIRBP were used.
Results: A progressive increase in the expression of CIRBP was observed from 12 to 48 hours, with statistically significant values after 24 and 48 hours compared to controls.
Conclusion: This experimental model demonstrated efficacy, accessibility, and economy to generate systemic hypothermia, which provides a novel range of therapeutic strategies. The increase in the expression of cold-inducible proteins in the rats’ spinal cords allows us to study the benefit of hypothermia at the molecular level for the first time, being of utmost importance for therapeutic studies in spinal cord injuries.

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How to Cite
Sarotto, A. J., Rey-Funes, M., Dorfman, V. B., Contartese, D., Larráyoz, I. M., Martínez, A., Toscanini, M. A., & Loidl, C. F. (2022). Expression of Cold-Inducible Proteins in Rat Spinal Cord Subjected to Systemic Hypothermia . Revista De La Asociación Argentina De Ortopedia Y Traumatología, 87(3), 393-403. https://doi.org/10.15417/issn.1852-7434.2022.87.3.1488
Section
Basic Research
Author Biographies

Aníbal José Sarotto, Laboratorio de Neuropatología Experimental, Instituto de Biología Celular y Neurociencia “Prof. E. De Robertis” (IBCN), School of Medicine, Universidad de Buenos Aires, CONICET, Autonomous City of Buenos Aires, Argentina

Laboratorio de Neuropatología Experimental, Instituto de Biología Celular y Neurociencia “Prof. E. De Robertis” (IBCN), School of Medicine, Universidad de Buenos Aires, CONICET, Autonomous City of Buenos Aires, Argentina

Manuel Rey-Funes, Laboratorio de Neuropatología Experimental, Instituto de Biología Celular y Neurociencia “Prof. E. De Robertis” (IBCN), School of Medicine, Universidad de Buenos Aires, CONICET, Autonomous City of Buenos Aires, Argentina

Laboratorio de Neuropatología Experimental, Instituto de Biología Celular y Neurociencia “Prof. E. De Robertis” (IBCN), School of Medicine, Universidad de Buenos Aires, CONICET, Autonomous City of Buenos Aires, Argentina

Verónica Berta Dorfman, Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y Diagnóstico (CEBBAD), Universidad Maimónides, Autonomous City of Buenos Aires, Argentina

Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y Diagnóstico (CEBBAD), Universidad Maimónides, Autonomous City of Buenos Aires, Argentina

Daniela Contartese, Laboratorio de Neuropatología Experimental, Instituto de Biología Celular y Neurociencia “Prof. E. De Robertis” (IBCN), School of Medicine, Universidad de Buenos Aires, CONICET, Autonomous City of Buenos Aires, Argentina

Laboratorio de Neuropatología Experimental, Instituto de Biología Celular y Neurociencia “Prof. E. De Robertis” (IBCN), School of Medicine, Universidad de Buenos Aires, CONICET, Autonomous City of Buenos Aires, Argentina

Ignacio M. Larráyoz, Biomarkers and Molecular Signaling Group, Center for Biomedical Research of La Rioja, Logroño, Spain

Biomarkers and Molecular Signaling Group, Center for Biomedical Research of La Rioja, Logroño, Spain

Alfredo Martínez, Angiogenesis Study Group, Center for Biomedical Research of La Rioja (CIBIR), Logroño, Spain

Angiogenesis Study Group, Center for Biomedical Research of La Rioja (CIBIR), Logroño, Spain

María Agustina Toscanini, Instituto NANOBIOTEC (UBA-CONICET), School of Pharmacy and Biochemistry, Universidad de Buenos Aires, CONICET, Autonomous City of Buenos Aires, Argentina

Instituto NANOBIOTEC (UBA-CONICET), School of Pharmacy and Biochemistry, Universidad de Buenos Aires, CONICET, Autonomous City of Buenos Aires, Argentina

César Fabián Loidl, Laboratorio de Neuropatología Experimental, Instituto de Biología Celular y Neurociencia “Prof. E. De Robertis” (IBCN), School of Medicine, Universidad de Buenos Aires, CONICET, Autonomous City of Buenos Aires, Argentina

Laboratorio de Neuropatología Experimental, Instituto de Biología Celular y Neurociencia “Prof. E. De Robertis” (IBCN), School of Medicine, Universidad de Buenos Aires, CONICET, Autonomous City of Buenos Aires, Argentina

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